Holding(s) in Company

NOTIFICATION OF MAJOR INTERESTS IN SHARES

1. Name of company

Alliance Trust PLC                                                           

2. Name of shareholder having a major interest

D C Thomson & Company Limited and John Leng & Company Limited                

3. Please state whether notification indicates that it is in respect of holding
of the shareholder named in 2 above or in respect of a non-beneficial interest
or in the case of an individual holder if it is a holding of that person's
spouse or children under the age of 18

Beneficial interests of the shareholders named in 2 above                    

4. Name of the registered holder(s) and, if more than one holder, the number of
shares held by each of them

D C Thomson & Company Limited             38,273,398                         
                                                                             
John Leng & Company Limited               11,872,650                         

 5. Number of shares / amount of stock acquired

By notification dated 26 June 2006 that following the merger of The Alliance 
Trust PLC and The Second Alliance Trust PLC that the interest in the issued  
share capital of the Company was as stated in 4 above.                       

6. Percentage of issued class

N/A                                                                          

7. Number of shares / amount of stock disposed

N/A                                                                          

8. Percentage of issued class

N/A                                                                          

9. Class of security

Ordinary shares of 2.5p                                                      

10. Date of transaction

N/A                                                                          

11. Date company informed

27 June 2006                                                                 

12. Total holding following this notification

Total holding disclosed 50,146,048                                           

13. Total percentage holding of issued class following this notification

Total holding disclosed 7.46%                                                

14. Any additional information



15. Name of contact and telephone number for queries

Ian Anderson, Assistant Company Secretary                                    
                                                                             
01382 201700                                                                 

16. Name and signature of authorised company official responsible for making
this notification

Ian Anderson, Assistant Company Secretary                                    

17. Date of notification

27 June 2006